Hi guys, I am doing Transfection with siRNAs, If I add serum with antibiotics accidentally, does that affect Transfection, as it is usually recommended to use...
What is your problem exactly? the amount of DNA? o how to measure DNA concentration? Martin 2009/9/26 SUZAN RAGHEB <drsuzanragheb@...> ... -- Martin...
Hello Thanks for the information...but can you help me little more by telling is it possible that the enzyme is showing optimum pH at alkaline side, the enzyme...
I want to check gene expresion for p53 with GADPH internal control... I have primer both for p53 and GADPH.... my question is which better to check gene...
Measure the spectrophotometric absorbance of the DNA sample at 260nm. Absorbance 1.0 at 260nm corresponds to 50 microgrms of ds DNS per mili liter. you can...
You have to tell us your beginning concentration so that we can tell you how much dilution you need to make from your stock DNA. ... From: SUZAN RAGHEB...
Dear all, I had previously isolated a parasite fluid (worm) and stored it at - 20 for almost 2 months now. The striking fact about this isolate is that when i...
Hi all, I am working on mutations and hv constructed phylogenetic trees but dont know how to xplain the exact seq relationships etc. Can u ppl help me to...
Histones are subject to a wide variety of posttranslational modifications including but not limited to, lysine acetylation, lysine and arginine methylation,...
Greetings to all, Dr.Sina was working at Canada in Acarolog and any body knows his contact details.I will be obliged to get the details. Thanking You, ...
Hi, Which software are you using I am also working on phylogenetic analysis. I found MEGA to be the best one. A book title fundamentals of molecular evolution...
Salmaan Sharif
salmaansharif@...
Oct 6, 2009 11:46 pm
8476
Hello, I'd like to extract DNA from samples to have total extracted DNA =200 microliter / sample also in the begining I want to extract DNA from different...
To make a reliable phylogenetic analysis 3 softwares are needed. 1. Clustal X 2. BioEdit 3. Phylip With clustal X you need to align your sequences. With...
Dear Hamid, I appreciate your consideration. I would be gratful if you could answer these qThe recepie that i used to prepare sample buffer is Sample loading...
elham rastegari
elham.rastegar@...
Oct 10, 2009 1:32 am
8479
Please could you inform us about the primer used for second DNA strand synthesis in Reverse transcription. Oligo dT primes the first DNA strand synthesis. ...
I hate to be one of those who takes their homework questions and tries to pawn them off on the web, but a number of these questions are NOT clearly explained...
International qPCR 2010 Symposium & Exhibition in Vienna 7th – 9th April 2010 The focus of the qPCR 2010 Event will be "The ongoing evolution of qPCR" Our...
Hello all, dose the measuring of DNA concentration" with spectrophotometer " undergo using a law ? how can I use this law: DNA conc in microgram/ml =OD...
Hi guys, I am working on MTOR inhibitors in prostate cancer. It would be really great to know and collaborate with some one who is working on MTOR as well, ...
Dear Suzane, the primers will not appear as a sharp band, it will appear as a very faint band, so I think it does not worth to evaluate your primers by this...
I extracted total RNA from cell culture and impurity 260/280= 2.03 ng/ul and 260/280=0.84 and data for cDNA from total RNA impurity 260/280=1.65 ng/ul and...
Dear friends, I have isolated protein from the samples of transgenic cotton carrying insecticidal genes with the purpose to quantify it. Meanwhile I have...
Hi If your protein in solution, store them in as small samples.you take one each when you require. Once you thaw it, you can refreeze it perhaps once or...
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